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UCSF Human Embryonic Stem Cell Research Center
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Protocols

It is the goal of the Human Embryonic Stem Cell Biology Program to provide the science community with up-to-date information on how to maintain thriving ES cells. Below are pdf files of protocols that we currently use to maintain our various stem cell lines.

Freezing & Thawing of hESCs
hESC Protocol
Irradiated MEFs
Passaging H9
Passaging HSF1
Passaging HSF6
Thawing MEFs